Journal: PLoS ONE

Article Title: Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures

doi: 10.1371/journal.pone.0132344

Figure Lengend Snippet: (a) O.D. values at 570 nm representing proliferation rates of L929 fibroblasts cultured in direct contact with DBS1, DBS2, DBS3, DBS4 granules, titanium (Ti) disc, and without granules (blank) (n = 3 per group) Statistically significant differences are indicated as *P<0.05 and compared with blank. (b) Morphology of L929 fibroblasts cultured in presence of the test materials cited above. Images were acquired after removal of the bone granules. Cells (blue arrows) grown in contact with DBS1 and DBS3 granules show a morphology similar to that of cells (black arrows) contacting the Ti disc or blank. The morphology of cells (red arrows) in contact with DBS2 and DBS4 granules is altered.

Article Snippet: The cytotoxicity of the decellularized bovine bone was evaluated in vitro using a mouse-derived established cell line of L929 fibroblasts (Cell bank Interlab Cell Line Collection, Genova, Italy).

Techniques: Cell Culture

Journal: Virology

Article Title: Lethal murine infection model for human respiratory disease-associated Pteropine orthoreovirus

doi: 10.1016/j.virol.2017.10.021

Figure Lengend Snippet: Neutralization activity (mean (95% CI)) of anti-PRV serum against Reoviridae viruses.

Article Snippet: Mouse fibroblast L929, lung adenocarcinoma A549, and rhesus monkey kidney MA104 cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) (Nacalai Tesque) supplemented with 5% fetal bovine serum (Gibco).

Techniques: Neutralization, Activity Assay

Journal: Polymers

Article Title: Antibacterial Chitosan Nanofiber Thin Films with Bacitracin Zinc Salt

doi: 10.3390/polym13071104

Figure Lengend Snippet: The cell viability of L929 fibroblasts after incubation with the thin films for 24 h (** p < 0.01).

Article Snippet: The cytotoxicity of the films was estimated from the viability of L929 fibroblasts (KAC Co., Ltd., Kyoto, Japan).

Techniques: Incubation

Journal: Annals of Medicine and Surgery

Article Title: Effects of heated tobacco products and conventional cigarettes on dental implant wound healing: experimental research

doi: 10.1097/MS9.0000000000000367

Figure Lengend Snippet: (A) Scratch wound-healing assay. Images from scratch assay experiments at different time-points (×40 magnification). Migrated cells are shown in red. (B) Number of L929 cell migration into a cell-free area. The number of L929 cells decreased on exposure to cigarette smoke extract from HTPs and CCs ( n =3). CCs, conventional cigarettes; HTPs, heated tobacco products.

Article Snippet: The L929 mouse fibroblast cell line was purchased from Sumitomo Pharma and cultured in MEM containing 5% foetal bovine serum, 100 U/ml penicillin, and 100 g/ml streptomycin (Thermo Fisher Scientific).

Techniques: Wound Healing Assay, Migration

Journal: International Journal of Nanomedicine

Article Title: Internal-External Homologous Drug-Loaded Exosome-Like Nanovesicles Released from Semi-IPN Hydrogel Enhancing Wound Healing of Chemoradiotherapy-Induced Oral Mucositis

doi: 10.2147/IJN.S508530

Figure Lengend Snippet: Characterization and biocompatibility of ORI/ENs loaded into semi-IPN hydrogel. ( A ) Schematic diagram depicting the preparation process of ORI/ENs/Gel. ( B ) The SEM images of ORI/ENs/Gel. ( C ) ORI release profiles from IPN and ORI/ENs/Gel. ( D ) The TEM images of ENs and ORI/ENs released from semi-IPN hydrogel. ( E ) Hemolysis test under different concentrations of ORI/ENs/Gel. ( F ) Effect of ORI/ENs/Gel on the cell viability of L929 cells. ( G ) Assessment of L929 cells proliferation and migration capacity. ( H ) Relative quantitative analysis of cell gap closure. * p < 0.05, vs the control group; # p < 0.05, vs ORI/ENs/Gel group, n=3.

Article Snippet: Mouse Fibroblast (L929) Cells were obtained from the Cold Spring Biotech Corp Company and incubated in DMEM medium (Solarbio Science & Technology, Beijing, China) supplemented with 1% penicillin-streptomycin as well as 10% FBS (BI Biological Industries, Israel) at 37°C in an environment of 5% CO 2 .

Techniques: Migration, Control

Journal:

Article Title: Nitric Oxide-Mediated Inhibition of the Ability of Rickettsia prowazekii To Infect Mouse Fibroblasts and Mouse Macrophagelike Cells

doi:

Figure Lengend Snippet: Effect of treatment of L929 cells with cytokines and NOS inhibitors on nitrite production and the initial infection of the cells with R. prowazekii a

Article Snippet: Mouse L929 cells (originally purchased from Flow Laboratories) were grown in Eagle minimal essential medium supplemented with 10% newborn bovine serum.

Techniques: Infection

Journal:

Article Title: Nitric Oxide-Mediated Inhibition of the Ability of Rickettsia prowazekii To Infect Mouse Fibroblasts and Mouse Macrophagelike Cells

doi:

Figure Lengend Snippet: Reduction of both suppression of the initial rickettsial infection and nitrite production in cultures of L929 cells treated with LK plus antibody against murine IFN-γ. Each bar represents the mean ± standard error of the mean for the number of rickettsiae per cell (expressed as a percentage of the value in the control cultures) in the following numbers of experiments: control, 2; 25% LK (IFN, 420 U/ml), 5; antibody against murine IFN-γ (105 U/ml), 2; and LK plus antibody, 5. In the control cultures, the percentage of cells infected was 89% ± 2%, the number of rickettsiae per infected cell was 2.9 ± 0.2, and the number of rickettsiae per cell was 2.6 ± 0.2.

Article Snippet: Mouse L929 cells (originally purchased from Flow Laboratories) were grown in Eagle minimal essential medium supplemented with 10% newborn bovine serum.

Techniques: Infection

Journal:

Article Title: Nitric Oxide-Mediated Inhibition of the Ability of Rickettsia prowazekii To Infect Mouse Fibroblasts and Mouse Macrophagelike Cells

doi:

Figure Lengend Snippet: Elimination of suppression of the initial rickettsial infection in treated L929 cell cultures by addition of oxyhemoglobin during the rickettsial infection. Each point represents the mean for the number of rickettsiae per cell (expressed as a percentage of the value in the control cultures) in at least two experiments, except for points representing cultures with 1 μM methemoglobin. The latter points represent the means of duplicate or triplicate determinations in a single experiment. The standard errors and standard deviations averaged 14% ± 2% of the mean values. In the control cultures, the percentage of cells infected was 86% ± 2%, the number of rickettsiae per infected cell was 3.3 ± 0.3, and the number of rickettsiae per cell was 2.9 ± 0.4. CONC, concentration; C, control; IT, IFN-γ (100 U/ml) + TNF-α (100 U/ml); MET, methemoglobin; OXY, oxyhemoglobin. LK was at 25%.

Article Snippet: Mouse L929 cells (originally purchased from Flow Laboratories) were grown in Eagle minimal essential medium supplemented with 10% newborn bovine serum.

Techniques: Infection, Concentration Assay

Journal:

Article Title: Nitric Oxide-Mediated Inhibition of the Ability of Rickettsia prowazekii To Infect Mouse Fibroblasts and Mouse Macrophagelike Cells

doi:

Figure Lengend Snippet: Inhibition of the initial rickettsial infection in L929 cells after exposure of isolated rickettsiae to various concentrations of NO. Before being added to L929 cells, samples of isolated rickettsiae were incubated at room temperature for 10 min with deoxygenated SPG-Mg equilibrated with air, deoxygenated SPG-Mg equilibrated with N2, or various concentrations of NO (diluted in deoxygenated SPG-Mg equilibrated with N2). Data are expressed as percentages of the corresponding values observed for rickettsial samples incubated with deoxygenated SPG-Mg equilibrated with air (air controls). In the L929 cell suspensions infected with these air control rickettsiae, the percentage of cells infected was 71% ± 9% and the number of rickettsiae per cell was 3.3 ± 1.3. Each point represents the mean ± standard deviation for at least three experiments.

Article Snippet: Mouse L929 cells (originally purchased from Flow Laboratories) were grown in Eagle minimal essential medium supplemented with 10% newborn bovine serum.

Techniques: Inhibition, Infection, Isolation, Incubation, Standard Deviation

Journal:

Article Title: Nitric Oxide-Mediated Inhibition of the Ability of Rickettsia prowazekii To Infect Mouse Fibroblasts and Mouse Macrophagelike Cells

doi:

Figure Lengend Snippet: Inhibition of the initial rickettsial infection in L929 cells after exposure of isolated rickettsiae to NO for various periods of time. Before being added to L929 cells, samples of isolated rickettsiae were incubated at room temperature for various time periods with deoxygenated SPG-Mg equilibrated with air, deoxygenated SPG-Mg equilibrated with N2, or 5 μM NO in deoxygenated SPG-Mg equilibrated with N2. Data are expressed as percentages of the corresponding values observed for rickettsial samples incubated with deoxygenated SPG-Mg equilibrated with air (air controls). In L929 cell suspensions infected with these air control rickettsiae, the average percentages of cells infected ranged from 52% to 71%, and the average numbers of rickettsiae per cell ranged from 3.3 to 4.9. Each point represents the mean ± standard deviation for at least two experiments.

Article Snippet: Mouse L929 cells (originally purchased from Flow Laboratories) were grown in Eagle minimal essential medium supplemented with 10% newborn bovine serum.

Techniques: Inhibition, Infection, Isolation, Incubation, Standard Deviation